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KMID : 1024520010100030239
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Journal of the Environmental Sciences
2001 Volume.10 No. 3 p.239 ~ p.245
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Dynamics of Microbial Community of Aerobic Biofilm Reactor using Rhodococcus sp. EL-GT and Activated Sludge for Phenol Wastewater Treatment
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Park Geun-Tae
Won Seong-Nae
Son Hong-Joo
Nam Gui-Sook
Lee Jae-Dong
Lee Sang-Joon
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Abstract
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This research was performed to investigate the dynamics of microbial community by RBC (Rotating Biological Contactor) using Rhodococcus sp. EL-GT and activated sludge. Cell counts revealed by DAPI were compared with culturable bacterial counts from nutrient agar. Colony counts on nutrient agar gave values 20~25% and 1~15% of cell counts (DAPI). The cell counts for the dynamics of bacterial community were determined by combination of in situ hybridization with fluorescently-labelled oligonyucleotide probes and epifluorescence microscopy. Around 90~80% of total cells visualized DAPI were also detected by the bacteria probe EUB 338. For both reactors proteobacteria belonging to the gamma subclass were dominant in the first stage (1 and 2 stage) and proteobacteria belonging to the gamma subclass were dominant in the last stage (3 and 4 stage).
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KEYWORD
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RBC, Microbial community, in situ hybridization, proteobacteria
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